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Immunofluorescence labeling of cyclin D1 (DCS-6), cyclin D2 (DCS-3), and cyclin D3 (DCS-22) in dog thyrocytes stimulated by different mitogenic treatments. Quiescent 4-d-old cells were stimulated for 20 h by TSH (1 mU/ml), EGF (25 ng/ ml)+serum (10%; ES), or remained in control condition (C). Exposure times of the photographs were the same for the different cell treatments, but were shorter for cyclin D3 than cyclin D1 and much longer for cyclin D2.
