Figure 5.
Double immunofluorescence labeling of cyclin D3 (detected using DCS-22), and PCNA used as a cell cycle marker in the same cells. Before fixation, 4-d-old dog thyrocytes were stimulated for 26 h with TSH (1 mU/ml), EGF (25 ng/ml)+serum (10%; ES), or remained quiescent in control medium (C) for this time. Arrowheads and large arrows show cells identified, respectively, in late G1 and S phase as described previously (Baptist et al., 1993, 1996). Small arrows show G0/G1 cells with a low PCNA staining.