Figure 11.
Wt-hCgB but not Δcys-hCgB or AT is stored in PC12 cells when expressed by recombinant vaccinia viruses. PC12 cells infected with either vv:wt-hCgB, vv: Δcys-hCgB, or vv:AT were pulse labeled for 5 min with [35S]sulfate, and chased for 90 min. Equal aliquots of cells and media collected at the end of the chase were analyzed, after immunoprecipitation (AT), preparation of heat stable fractions from cell homogenates (hCgB, cells) or directly (hCgB, media), by SDS-PAGE and phosphoimaging. Storage efficiencies of labeled proteins in the cell are expressed as percentage of total (sum of cells plus medium). Error bars show SEM of three independent experiments.