Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Mar 23;140(6):1331–1346. doi: 10.1083/jcb.140.6.1331

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Storage efficiencies of wt-hCgB, Δcys-hCgB, and AT transiently transfected in PC12 cells. PC12 cells transfected with wt-hCgB, Δcys-hCgB, or AT were pulse labeled for 5 min with [³⁵S]sulfate and chased for the indicated times. Equal aliquots of cells and media collected at the end of the chase were analyzed by immunoprecipitation and SDS-PAGE followed by phosphoimaging. (A) Autoradiograms of cell lysates (lanes C) and media (lanes M) at the end of the pulse (0 min of chase) and after 90 min of chase. (Bracket: hCgB; diamond: hCgB fragments; asterisk: AT). Note the slightly slower mobility of Δcys-hCgB as compared to wt-hCgB. (B) Secretion of wt-hCgB, Δcys-hCgB, and AT after different chase times. (C) Storage efficiencies were determined after 90 min of chase (four independent experiments). Error bars show standard deviation. In B and C, the labeled protein in the medium (B) and in the cell (C) is expressed as percent of total (sum of cell plus medium).

Storage efficiencies of wt-hCgB, Δcys-hCgB, and AT transiently transfected in PC12 cells. PC12 cells transfected with wt-hCgB, Δcys-hCgB, or AT were pulse labeled for 5 min with [³⁵S]sulfate and chased for the indicated times. Equal aliquots of cells and media collected at the end of the chase were analyzed by immunoprecipitation and SDS-PAGE followed by phosphoimaging. (A) Autoradiograms of cell lysates (lanes C) and media (lanes M) at the end of the pulse (0 min of chase) and after 90 min of chase. (Bracket: hCgB; diamond: hCgB fragments; asterisk: AT). Note the slightly slower mobility of Δcys-hCgB as compared to wt-hCgB. (B) Secretion of wt-hCgB, Δcys-hCgB, and AT after different chase times. (C) Storage efficiencies were determined after 90 min of chase (four independent experiments). Error bars show standard deviation. In B and C, the labeled protein in the medium (B) and in the cell (C) is expressed as percent of total (sum of cell plus medium).