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. 1998 Mar 23;140(6):1331–1346. doi: 10.1083/jcb.140.6.1331

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Time course of arrival of newly synthesized hCgB at the trans-Golgi. Top: PC12 cells infected with 100 pfu/cell vv:wt-hCgB for 6.5 h were pulse labeled for 3 min with [³⁵S]methionine and chased for the indicated times. At the end of various chase periods, a heat stable protein fraction was prepared from the cells and analyzed by SDS-PAGE and fluorography. trans-Golgi form, arrow; rough ER form of wt-hCgB, arrowhead. Bottom: The trans-Golgi form of [³⁵S]methionine-labeled hCgB is expressed as percentage of total (sum of rough ER and trans-Golgi forms of hCgB). Arrowhead and dashed line, t _1/2 of ER to trans-Golgi transport.

Time course of arrival of newly synthesized hCgB at the trans-Golgi. Top: PC12 cells infected with 100 pfu/cell vv:wt-hCgB for 6.5 h were pulse labeled for 3 min with [³⁵S]methionine and chased for the indicated times. At the end of various chase periods, a heat stable protein fraction was prepared from the cells and analyzed by SDS-PAGE and fluorography. trans-Golgi form, arrow; rough ER form of wt-hCgB, arrowhead. Bottom: The trans-Golgi form of [³⁵S]methionine-labeled hCgB is expressed as percentage of total (sum of rough ER and trans-Golgi forms of hCgB). Arrowhead and dashed line, t _1/2 of ER to trans-Golgi transport.