Table I.
Quantification of Budding and Micronucleation
| BrdU labeling in: | Frequency of DM+buds that belong to each type | |||||
|---|---|---|---|---|---|---|
| Type | DM+buds | Nuclei | ||||
| 1 | + | + | 26/60 (43%) | |||
| 1′ | ± | + | 3/60 (5%) | |||
| 2 | − | + | 9/60 (15%) | |||
| 2′ | − | ± | 12/60 (20%) | |||
| 3 | − | − | 10/60 (17%) | |||
Nuclei from COLO 320DM were pulse labeled with BrdU and then analyzed for BrdU incorporation and DMs as described in Fig. 6. Nuclei were observed using epifluorescence microscopy. 60 nuclei with DM+buds were classified according to whether they labeled with BrdU (classes 1, 1′), whether the nuclei to which they were attached labeled with BrdU (classes 2, 2′), or whether neither bud nor nucleus labeled with BrdU (class 3). The number of nuclei belonging to each type were scored and expressed as nuclei in that class/total number scored.