Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Mar 23;140(6):1285–1295. doi: 10.1083/jcb.140.6.1285

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Replicon clusters are stable over many cell generations. Cells synchronized at the beginning of S phase were grown for 20 min in medium containing BrdU and then 5 or 10 d in medium. Sites of BrdU incorporation were indirectly immunolabeled with FITC 5 (A) or 10 d (B–E) later. A shows a single cell with many clusters of labeled foci and (B–D) isolated clusters of labeled foci, the only labeled sites within three different cells. Foci are scattered along metaphase chromosomes in the same preparations (E); double exposure shows that foci are restricted to only one chromatid. The foci of 200 discrete clusters immunolabeled 5–10 d after incorporating BrdU in cells at the beginning of S phase (F), or unsynchronized cells (G) were counted and their distribution (open bars) compared with foci of 100 metaphase chromosomes (closed bars). Bars: (A) 2 μm; (B–E) 1 μm.

Replicon clusters are stable over many cell generations. Cells synchronized at the beginning of S phase were grown for 20 min in medium containing BrdU and then 5 or 10 d in medium. Sites of BrdU incorporation were indirectly immunolabeled with FITC 5 (A) or 10 d (B–E) later. A shows a single cell with many clusters of labeled foci and (B–D) isolated clusters of labeled foci, the only labeled sites within three different cells. Foci are scattered along metaphase chromosomes in the same preparations (E); double exposure shows that foci are restricted to only one chromatid. The foci of 200 discrete clusters immunolabeled 5–10 d after incorporating BrdU in cells at the beginning of S phase (F), or unsynchronized cells (G) were counted and their distribution (open bars) compared with foci of 100 metaphase chromosomes (closed bars). Bars: (A) 2 μm; (B–E) 1 μm.