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. 1998 Mar 23;140(6):1383–1393. doi: 10.1083/jcb.140.6.1383

Figure 2.

Figure 2

Detection of actinin-4 protein and mRNA. (A) Immunoblot analysis of actinin-4 in HFK and various human cancer cell lines with mAb NCC-Lu-632. Actinin-4 protein was detected in cell lysates from HFK (lane 1), lung cancer cell lines Lu-65 (lane 2) and PC-10 (lane 3), vulvar cancer cell line A-431 (lane 4), and esophageal cancer cell lines TE 4 (lane 5), TE 6 (lane 6), TE 7 (lane 7), TE 10 (lane 8), and TE 11 (lane 9). Molecular masses (in kD) are shown on the left. (B) Expression of actinin-4 mRNA in normal human tissues. Human multiple tissue Northern blots I and II (CLONTECH Laboratories) were hybridized with an actinin-4–specific oligonucleotide. Each lane contains 2 μg of poly(A)+ RNA of human adult tissues: heart (lane 1), brain (lane 2), placenta (lane 3), lung (lane 4), liver (lane 5), skeletal muscle (lane 6), kidney (lane 7), pancreas (lane 8), spleen (lane 9), thymus (lane 10), prostate (lane 11), testis (lane 12), ovary (lane 13), small intestine (lane 14), colon (lane 15), and peripheral blood leukocytes (lane 16). Molecular masses (in kb) are shown on the left side.