Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Mar 23;140(6):1347–1356. doi: 10.1083/jcb.140.6.1347

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Cloning of VID24. (A) A 36-kb DNA sequence flanking LYS2 was obtained from the American Type Culture Collection, digested by either XbaI (X) or BamHI (B), and then subcloned into a yeast centromeric vector to generate pMC1-pMC5. These plasmids were introduced into the vid24-1 mutant and complementation of FBPase degradation defect was examined by Western blotting (right-hand column). pMC5 complemented the degradation defect and was subcloned to yield pMC5-1 and pMC5-2. pMC5-2, but not pMC5-1, complemented the defect. (B) The VID24 gene was deleted by replacing the entire gene with TRP1. The deleted gene was introduced into wild-type cells and the transformants were selected on the tryptophan drop-out plates. The deletion was confirmed by PCR reactions. (C) The degradation of FBPase in wild-type, vid24-1, and the Δvid24:: TRP1 strains after glucose shift for 0–180 min.