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. 1998 Mar 23;140(6):1347–1356. doi: 10.1083/jcb.140.6.1347

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Vid24p is synthesized in response to glucose. Wild-type cells expressing the HA-Vid24p were cultured and shifted to glucose for 0, 20, 40, 60, and 100 min at 22°C. The induction of HA-Vid24p and the degradation of FBPase in response to glucose were examined by Western blotting with anti-HA and anti-FBPase antibodies. Lanes 1–5, untransformed wild-type cells were shifted to glucose for 0–100 min. Lanes 6–10, wild-type cells expressing the HA-Vid24p were shifted to glucose for 0–100 min in the absence of cycloheximide. Lanes 11 and 12, wild-type cells expressing the HA-Vid24p were shifted to glucose for 0 and 100 min in the presence of 10 μg/ml of cycloheximide.