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. 1998 Mar 23;140(6):1347–1356. doi: 10.1083/jcb.140.6.1347

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FBPase is recovered in the high speed pellet in the vid24-1 mutant. pep4 and vid24-1 cells were grown in YPKG and transferred to YPD containing 2% glucose for 60 min at 22°C. Cell lysates were separated into medium speed supernatant (T), high speed supernatant (S) and high speed pellet (P) as described in Materials and Methods. The distribution of FBPase in pep4 (a and b) and vid24-1 cells (c and d) at t = 0 and t = 60 min was detected by Western blotting. The distribution of G-6-P dehydrogenase in the pep4 (e and f) and vid24-1 cells (g and h) at t = 0 min and t = 60 min was detected by Western blotting with G-6-P dehydrogenase antibodies.