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. 1998 Mar 9;140(5):1265–1276. doi: 10.1083/jcb.140.5.1265

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Expression of IRF proteins. (A) Extracts were prepared from undifferentiated and retinoic acid–treated P19 cells (15 μg), C2C12 myoblasts (15 μg), and embryonic and adult skeletal muscle (30 μg), separated by SDS-PAGE, transferred to nitrocellulose membrane, and immunoblotted with anti–IRF-2 antisera. (B) 15 μg of whole cell extracts prepared from untreated and IFN-γ-treated (3 h, 1000 U/ml) NIH 3T3 and C2C12 cells were separated by SDS-PAGE, transferred to nitrocellulose membrane, and immunoblotted with anti–IRF-1 antisera. NS denotes a nonspecific complex.