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. 1998 Mar 9;140(5):991–1002. doi: 10.1083/jcb.140.5.991

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INCENP open reading frame and deletion mutants used in this study. (Top) Map of the INCENP cDNA showing the position of important restriction sites. Below this are the various INCENP constructs used in this study. The region of putative coiled-coil (Berger et al., 1995) is stippled. (Bottom) Known functional regions required for chromosome binding and centromere targeting (this study) and for transfer to the spindle and microtubule binding (Mackay et al., 1993). The results of expression of each species in cultured cells are summarized on the right. Because our molecular cloning analysis (Mackay et al., 1993) revealed that the two INCENP polypeptides are likely to be the alternatively spliced products of a single gene, we now refer to this gene and both of its products as INCENP.