Figure 4.

K_d ratio of elution behavior for RCAM RNase:RNase A, and upper and lower bounds of Gibbs energies of transfer of RCAM RNase from water to 1 M of the solutes indicated. The K_d ratio of RCAM RNase:RNase A is proportional to the Stokes radius ratio of RNase A:RCAM RNase. Filled bars represent the K_d ratio data and unfilled bars are the transfer Gibbs energies of RCAM RNase (see text) representing the upper and lower bounds of transfer Gibbs Energies, ▵G_{tr ub} and ▵G_{tr lb} from Eqs. 2 and 3. Errors in K_d ratios estimated from elution volume data are less than 1%.