Table II.
Effects of Acylation Site Mutations on Vacuole Inheritance in Zygotes
|
||||||
|---|---|---|---|---|---|---|
| Cross | Normal transfer | Transfer from labeled parent to bud only | No transfer from labeled parent | |||
| VAC8 × Δvac8 | 7% | 87% | 6% | |||
| vac8-2 × Δvac8 | 14% | 75% | 11% | |||
| vac8-3 × Δvac8 | 2% | 7% | 91% | |||
| vac8-4 × Δvac8 | 2% | 3% | 95% | |||
| VAC8 ×VAC8 | 75% | 15% | 11% | |||
| Δvac8 × VAC8 | 7% | 3% | 90% | |||
| Δvac8 × vac8-2 | 2% | 9% | 89% | |||
| Δvac8 × vac8-3 | 4% | 5% | 91% | |||
| Δvac8 × vac8-4 | 2% | 4% | 94% |
The strains listed on the left were labeled with FM4-64. After removing FM4-64 from the medium, the labeled cells were mixed with an equal number of cells of the opposite mating type. Cultures were incubated with shaking at 23°C for 4–4.5 h. Cells were examined by fluorescence microscopy and scored for the presence of labeled vacuoles. At least 50 zygotes were scored in each cross.