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. 1998 Apr 6;141(1):199–208. doi: 10.1083/jcb.141.1.199

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ImmunoEM colocalization of ZO-1 (10 nm gold) and ZO-3 (5 nm gold) on membranes isolated from MDCK/Z3 cells. (a–c) Three different images of labeling are displayed, showing that ZO-3 and ZO-1 are colocalized at sites of tight junction membrane contact, while all other membranes, including a desmosome (D) in b, are free of label. Bars, 100 nm.