Table II.
Surface Density of Caveolae in Antibody-injected Hepatocytes
| Injected antibody | Caveola density* | Range | n | Total surface membrane | Fold increase | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| per μm | caveolae per μm | μm | ||||||||
| HI Ab | 0.93 ± 0.28 | 0.33–4.46 | 23 | 295.7 | 1.0 | |||||
| αDyn Ab | 2.18 ± 0.30‡ | 0.74–7.91 | 34 | 307.2 | 2.3 |
Hepatocytes were injected with heat-inactivated antibodies as controls (HI Ab) or the anti-dynamin antibodies (αDyn Ab; anti-Pan65, n = 27 and anti-Dyn2T, n = 7) then fixed and processed for electron microscopy using ruthenium red to stain the plasma membranes as in Fig. 5, d and e. The surface density of caveolae in antibody-injected hepatocytes was determined from electron micrographs of complete cell profiles (n) for each set of injections.
*
Values are expressed as the median density ± SEM.
‡
Using the Wilcoxin rank sum analysis this value differs significantly from the control value (P < 0.001; two-tailed test).