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. 1998 Apr 6;141(1):187–197. doi: 10.1083/jcb.141.1.187

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Effects of Rnd1 expression on actin filament assembly. Serum-starved confluent Swiss 3T3 fibroblasts were injected with a pRK5 vector expressing Rnd1 (A–F) or myc-tagged Rnd3/ RhoE (G and H). 1.5–2.5 h later, cells were either left unstimulated for a further 30 min (A and B), stimulated with LPA (100 ng/ml) for 30 min (C, D, G, and H), or stimulated with PDGF (5 ng/ml) for 20 min (E and F) before fixation. Permeabilized cells were stained to show Rnd1 expression (B, D, and F), myc-tagged Rnd3/RhoE expression (H), and actin filaments (A, C, E, and G). Bar, 50 μm.