Figure 10.

Approximately 1 ng of laminin-5 (LN-5), fibronectin (FN), and bovine serum albumin (BSA) were dotted onto nitrocellulose (left). After blocking, the membranes were incubated with 20 μg/ml of tPA, plasminogen (Pg), or uPA overnight at 4°C (top). Bound protein was then detected with the appropriate antibody. Note that tPA binds both fibronectin and laminin-5, whereas plasminogen binds only laminin-5. uPA binds both BSA and fibronectin and also shows some binding to laminin-5.