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. 1998 Apr 6;141(1):255–265. doi: 10.1083/jcb.141.1.255

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SCC12 cells were plated onto pp126 ECM, pp126 ECM that had been treated with plasmin (Pm) (1 μg/ml for 90 min), MCF-10A ECM, affinity-purified pp126 laminin-5, affinity-purified pp126 laminin-5 treated with plasmin (Pm) (1 μg/ml for 90 min), or pp126 ECM treated with 10 μg/ml tPA overnight. After 1 h the motility of the SCC12 cells was assayed by video microscopy and then quantitated as the average of the total displacements of each cell over a 2-h period. For each substrate, we performed at least three trials in which a total of at least ninety cells were evaluated. Note that SCC12 cells show similar motility on pp126 ECM and affinity-purified pp126 laminin-5. They show less motility on plasmin-treated pp126 ECM, plasmin-modified purified pp126 laminin-5, MCF-10A ECM, and pp126 ECM treated with tPA. Error bars indicate standard deviations.