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. 1998 Apr 6;141(1):255–265. doi: 10.1083/jcb.141.1.255

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Approximately 10 μg of MCF-10A and pp126 ECM and 5 μg of laminin-1 were processed for SDS-PAGE and then separated polypeptides were transferred to nitrocellulose. The nitrocellulose sheet was then subjected to immunoblotting using a monoclonal antibody against the β1 subunit of laminin. This antibody recognizes a 200-kD protein in laminin-1 but no obvious polypeptides in MCF-10A or pp126 ECM.