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. 1998 Apr 6;141(1):255–265. doi: 10.1083/jcb.141.1.255

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Approximately 10 μg of untreated MCF-10A cell matrix (lane 1), untreated pp126 cell matrix (lane 2), or pp126 cell matrix treated for 16 h with tPA (∼50 μg of matrix incubated for 16 h with 1 ml of tPA at a concentration of either 5 or 10 μg/ml) (lanes 3 and 4) were subjected to SDS-PAGE on 6% gels, transferred to nitrocellulose, and then processed for immunoblotting using the laminin-5 α3 subunit antibody 10B5. In untreated pp126 cell matrix, the α3 subunit shows a molecular weight of 190 kD (lane 2) (refer to Fig. 2). The α3 chain from tPA-treated pp126 matrices comigrates with the α3 chain of MCF-10A matrix at 160 kD (lanes 1, 3, and 4). Note the small amount of residual 190-kD polypeptide in the 5 μg/ml tPA-treated pp126 matrix (lane 3). Molecular weight standards are shown to the left.