Table II.

Comparison of Probe Position in the Nucleus for Paired and Unpaired Sites

				Radial position ± SD				Vertical position ± SD				Distance to nuclear envelope ± SD
Probe		No. of nuclei		Paired		Unpaired		Paired		Unpaired		Paired		Unpaired
								μm
AACAC		46		1.38 ± 0.61		1.50 ± 0.48		3.06 ± 1.00		3.55 ± 0.54		0.62 ± 0.56		0.47 ± 0.59
Rsp		29		1.41 ± 0.83		1.33 ± 0.54		1.00 ± 0.77		1.36 ± 0.63		1.39 ± 1.02		1.80 ± 1.95
AATAG		61		0.83 ± 0.40		1.22 ± 0.61		2.96 ± 0.46		2.84 ± 0.92		0.99 ± 0.38		0.79 ± 0.90
96-45		53		1.06 ± 0.44		1.17 ± 0.51		3.18 ± 0.63		3.15 ± 0.82		1.42 ± 0.59		1.25 ± 1.45
Histone		100		0.95 ± 0.32		0.79 ± 0.34		0.53 ± 0.42		0.45 ± 0.31		1.60 ± 0.48		1.40 ± 0.48
09-93		38		1.72 ± 0.16		1.52 ± 0.61		0.18 ± 0.21		0.00 ± 0.65		0.51 ± 0.31		0.71 ± 1.0
44-93		49		1.12 ± 0.63		1.57 ± 0.53		0.00 ± 0.47		0.57 ± 0.84		1.30 ± 1.02		0.85 ± 1.10
02-82		41		2.11 ± 0.73		1.54 ± 0.62		0.24 ± 0.52		−0.31 ± 1.15		0.59 ± 0.62		0.87 ± 1.11
16-89		109		1.57 ± 0.56		1.70 ± 0.59		−1.21 ± 2.72		−1.20 ± 2.66		1.01 ± 0.72		0.83 ± 1.08
14-92		85		1.76 ± 0.76		1.92 ± 0.64		−1.53 ± 3.40		−1.67 ± 3.54		0.83 ± 0.91		0.70 ± 1.03
32-95		47		1.20 ± 0.46		1.11 ± 0.54		−1.22 ± 2.51		−1.11 ± 2.31		0.28 ± 0.37		0.34 ± 0.64

Average radial positions, vertical (Rabl) positions and average distance from the nuclear envelope were measured for all the sites to determine whether any differences existed between paired and unpaired loci. Measurements were taken from cycle 14 nuclei, 7–9 μm in length. Probe order is listed from centromere proximal to distal.