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. 1998 Apr 6;141(1):61–70. doi: 10.1083/jcb.141.1.61

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Purification of Sec23–24 from rat liver cytosol. The Sec23–24 complex was purified from rat liver cytosol as described in the Materials and Methods. A and B illustrate typical elution profiles from the DEAE and HAP chromatography steps, respectively. (C), A silver-stained gel of representative pooled fractions from crude cytosol (3.3 μg) (lane a), ammonium sulfate precipitate (3 μg) (lane b), S-300–Sepharose (2.8 μg) (lane c), DEAE (0.7 μg) (lane d), and HAP (0.2 μg) (lane e) columns. The asterisks in e, partial proteolytic breakdown products of Sec24 based on Western blotting. Molecular markers are indicated in the left margin of C (kD).

Purification of Sec23–24 from rat liver cytosol. The Sec23–24 complex was purified from rat liver cytosol as described in the Materials and Methods. A and B illustrate typical elution profiles from the DEAE and HAP chromatography steps, respectively. (C), A silver-stained gel of representative pooled fractions from crude cytosol (3.3 μg) (lane a), ammonium sulfate precipitate (3 μg) (lane b), S-300–Sepharose (2.8 μg) (lane c), DEAE (0.7 μg) (lane d), and HAP (0.2 μg) (lane e) columns. The asterisks in e, partial proteolytic breakdown products of Sec24 based on Western blotting. Molecular markers are indicated in the left margin of C (kD).