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Cross-linking of TF and IgA tracers internalized from the same and from opposite sides of the monolayer at low temperature. Polarized MDCK cells expressing TR and pIgR were incubated with radio-labeled or HRP-conjugated tracers from either the same or opposite sides of the monolayer, as indicated, for 2 h at 20°C. DAB cross-linking was then performed, as described in Materials and Methods. Results are means ± SD of three or four observations.
