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. 1998 May 4;141(3):839–847. doi: 10.1083/jcb.141.3.839

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SDS-PAGE and immunoblotting of purified osteoadherin. An extract of the bone mineral compartment, i.e., with guanidine hydrochloride/EDTA (A, left lane) and osteoadherin purified from bovine bone (A, right lane) were applied to an SDS 4–12% polyacrylamide gel after reduction with mercaptoethanol. The gel was stained with Coomassie blue (A). An identical gel was transferred to nitrocellulose (47) and immunoblotted with a rabbit antiserum against the purified bovine osteoadherin (B). One band was detected in the guanidine hydrochloride EDTA extract (B, left lane) corresponding to the purified osteoadherin (B, right lane).