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. 1998 May 4;141(3):839–847. doi: 10.1083/jcb.141.3.839

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Immunolocalization of osteoadherin in the bovine fetal rib growth plate. The chondrocostal growth plate of a bovine fetus was sectioned on a cryostat in 5-μm sections without prior decalcification. An affinity-purified antiserum against bovine osteoadherin was used for immunostaining. The sections were incubated with antibodies against osteoadherin (A–B) or with preimmune serum (C) followed by a biotinylated second antibody. The sections were incubated with Vectastain ABC reagent and developed in peroxidase solution. (A) Strong specific staining for the osteoadherin in the primary spongiosa in the fetal rib growth plate. (B) Staining of bone trabeculae from the same section as in A with higher magnification. (C) The control with the preimmune serum where no staining can be detected. Bar, 200 μm.

Immunolocalization of osteoadherin in the bovine fetal rib growth plate. The chondrocostal growth plate of a bovine fetus was sectioned on a cryostat in 5-μm sections without prior decalcification. An affinity-purified antiserum against bovine osteoadherin was used for immunostaining. The sections were incubated with antibodies against osteoadherin (A–B) or with preimmune serum (C) followed by a biotinylated second antibody. The sections were incubated with Vectastain ABC reagent and developed in peroxidase solution. (A) Strong specific staining for the osteoadherin in the primary spongiosa in the fetal rib growth plate. (B) Staining of bone trabeculae from the same section as in A with higher magnification. (C) The control with the preimmune serum where no staining can be detected. Bar, 200 μm.