Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 May 4;141(3):839–847. doi: 10.1083/jcb.141.3.839

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Affinity purification and immune precipitation of integrins binding to osteoadherin. A detergent extract of surface- iodinated MG 63 osteosarcoma cells was applied to a control agarose followed by OSAD agarose. Consecutive fractions were collected after adding EDTA to the elution buffer. The receptor-containing fractions were pooled. Aliquots of the pooled fractions were immunoprecipitated with polyclonal antibodies against the integrin subunits α_v and β₃, respectively. The pooled eluate and precipitated proteins were electrophoresed on SDS-PAGE (4–12%) under reducing conditions, and were visualized with the phosphor image analyzer Bas2000 (Fuji). (A) Total cell extract. (B) The left lane shows the EDTA eluate from the OSAD column. The middle lane shows immunoprecipiation with a peptide antiserum against the cytoplasmic tail of β₃. The right lane presents precipitation with a monoclonal antiserum specific for the β₃ subunit. (C) The left lane shows precipitation with a polyclonal antiserum against the cytoplasmic tail of α_v. The right lane shows immunoprecipitation with a polyclonal antiserum against the α_v subunit.