Figure 9.

Dual localization of B4 and CC3.4 domains in apoB in STP-O–permeabilized cells. HepG2 cells were permeabilized with STP-O and probed simultaneously with monoclonal CC3.4 (1/ 100) and affinity-purified rabbit anti-B4 peptide antibody (1/25). After washing, the cells were incubated with the appropriate secondary antibodies conjugated with different fluorochromes and examined at 570 nm (A, green; CC3.4 monoclonal) and 680 nm (B, red; anti-B4 peptide). The digital images were superimposed, and significant overlap is denoted by yellow (C).