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. 1998 May 4;141(3):553–566. doi: 10.1083/jcb.141.3.553

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Characterization of two NRP/B forms. A nuclear pellet of neurons was prepared and resuspended in SDS loading buffer (lane 1). In addition, total cell lysates of primary neurons (500 μg) were immunoprecipitated with NRP/B monoclonal antibody and analyzed by SDS-PAGE. Both samples were blotted with VD2 monoclonal NRP/B antibody (dilution 1:1,000).