Table II.

Quantitative Analysis of Neurite Outgrowth in Sense- and Antisense-treated Hippocampal Neuron Cultures

Oligonucleotide		Percentage of cells with neurites		Neurite length
				μm
Control		92 ± 4		115 ± 11
Sense		90 ± 4		116 ± 9
Scramble		88 ± 3		120 ± 7
Antisense		21 ± 2*		44 ± 5

Embryonic day 18 hippocampal neuron cultures were prepared as described in Materials and Methods. Neurite lengths and the percentages of cells with neurites were measured 48 h after treatment with sense, scramble, and antisense oligonucleotides against NRP/B mRNA. A total of 150 cells from three repeated experiments for each condition were counted. Data are the means ± SE values.  

^* Significantly inhibited neurite outgrowth compared to sense treatment (P < 0.005).