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Schematic diagram of plasmid pMSG-RPS3a. A human RPS3a cDNA, containing the complete protein coding region plus 5′ and 3′ UTRs and expressed under the control of the MMTV LTR, and the SV-40 early promoter–driven gpt gene, are present in the same orientation. The 950-bp EcoRI fragment containing the RPS3a cDNA and the 950-bp HindIII–AatII fragment of the gpt gene were used as probes for Southern and Northern blot analyses.
