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. 1998 May 4;141(3):741–753. doi: 10.1083/jcb.141.3.741

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Determination of stable integration by Southern blot analysis of genomic DNA. (a) SmaI- and EcoRV-digested DNA of parental NIH 3T3 cells and P-7 and P-12 transfectants, and (b) EcoRV-digested DNA of NIH 3T3 and S-8 and S-12 transfectants, were hybridized with the gpt probe. (c) HindIII-digested DNA of NIH 3T3, S-8, and S-12 cells, and (d) EcoRV-digested DNA of NIH 3T3 and S-12 cells, were hybridized with the RPS3a cDNA probe. The fragments derived from transfected RPS3a/gpt sequences are shown by ◂. Sizes of HindIII- or PstI-digested λ DNA fragments (in kb) are shown at the left of each panel.