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. 1998 May 4;141(3):741–753. doi: 10.1083/jcb.141.3.741

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Northern blot analysis of RPS3a expression. RNA was isolated from parental NIH 3T3 cells cultured in normal medium (lane 1), and from P-12 and S-12 cells cultured in gpt medium (lanes 2 and 5), switched to Dex medium for 2 d (lanes 3 and 6), and then switched back to gpt medium for 1 d (lanes 4 and 7). 8 μg of RNA was electrophoresed on a 1.6% agarose gel and used to prepare a Northern blot that was hybridized with the RPS3a cDNA probe. Endogenous RPS3a mRNAs were detected in all samples. The longer exogenous RPS3a transcripts were induced in S-12 cells switched from gpt to Dex medium (lane 6), and then inhibited when cells were switched back to gpt medium (lane 7). Equalization of RNA content was checked by visualizing 28S and 18S rRNAs on the ethidium bromide–stained RNA gel.