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Comparison of the concentration-dependent effects of filipin on CT activity in CaCo-2 (•), A431 (○) and Jurkat (□) cells. Cells were treated with increasing concentrations of filipin for 1 h at 37°C, then incubated an additional 2 h at 37°C with 0.03 nM CT and assayed for cAMP accumulation as described in Materials and Methods.
