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Osteopontin induces nuclear translocation of the p65 subunit. Cells were plated on osteopontin (A)- or polylysine (B)- coated surfaces in serum-free medium. 2 h after plating, cells were fixed and stained with a polyclonal anti-p65 subunit antibody. Bar, 10 μM. (C) Staining of cells plated on osteopontin with control nonimmune serum. Bar, 20 μM.
