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. 1998 May 18;141(4):917–927. doi: 10.1083/jcb.141.4.917

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Effect of CT and LTIIb chimeric toxins on cAMP-dependent Cl⁻ secretion in polarized T84 monolayers. (A) ΔIsc (peak Isc mean ± SEM, 120 min after toxin addition corrected for baseline currents) for wt LTIIb(S193M), chimeric toxin 187, or chimeric toxin 184 (LTIIb_A/CT_B) applied to apical reservoirs of T84 cell monolayers in the presence or absence of purified GM1, GD1a, rCT B subunit, and rLTIIB as competitive inhibitors. ANOVA, P = 0.0001. * indicates conditions significantly different from chimera 184 alone (third column) at P ≤ 0.05 by multiple comparison procedures. (B) ΔIsc (peak Isc mean ± SEM, 120 min after toxin addition corrected for baseline currents) for wt rCT and chimeric toxin 179 (CT_A/LTIIb_B) to apical reservoirs of T84 cell monolayers in the presence or absence of GM1 or rCT B subunit as competitive inhibitors. ANOVA, P = 0.0001. * indicates conditions significantly different from rCT alone (third column) at P ≤ 0.05 by multiple comparison procedures. In all studies, mean ± SEM baseline currents were 1.1 ± 0.2 μA/cm², n = 25.