Figure 4.

Sucrose density gradient profile of membrane plus matrix. The membrane plus matrix fraction from flagella of wild-type cells was fractionated on an 11-ml 5–20% sucrose gradient in 10 mM Hepes, pH 7.2. The Coomassie-stained gel of the gradient profile shows that the putative IFT particle polypeptides cosediment at 16 S (highlighted by both sets of arrowheads with their apparent mobilities listed on the left).