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. 1998 May 18;141(4):929–942. doi: 10.1083/jcb.141.4.929

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Patching of GM1/cholera toxin with PLAP and hTfR del 5–41 in Jurkat T–lymphoma cells. FITC-CTx conjugate was used to patch and stain GM 1 (green). Respective mAbs against PLAP or hTfR were used followed by incubation with rhodamine-coupled secondary antibodies (red). PLAP (A) and GM1 (B) show extensive copatching (merge in C). Patched hTfR del 5–41 (D) and GM1 patches (E) are largely exclusive (merge in F). Bar, 5 μm.