Figure 1.

Colocalization of phosphotyrosine with β-catenin and p120-Cas in the cortical cytoskeleton of differentiating keratinocytes. Mouse primary keratinocytes in low calcium medium or at 9 h of calcium exposure were pre-extracted in 0.2% Triton X-100 buffer before paraformaldehyde fixation as described in Materials and Methods. Cells were double stained with anti-phosphotyrosine antibodies and FITC-conjugated secondaries (green) and antibodies against either β-catenin (top panels) or p120-Cas (bottom panels), and Texas red–conjugated secondaries (red). Samples were analyzed by confocal microscopy and green and red images (small panels) were superimposed (large panels), so that sites of staining overlap are visualized as yellow. Bars: (large panels) 15 μm; (small panels) 33 μm.