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. 1998 Jun 15;141(6):1323–1333. doi: 10.1083/jcb.141.6.1323

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Subcellular fractionation of infected Vero cells containing transgenic T. gondii Vero cells infected with T. gondii expressing either BAP, BAP-GPI, or BAP-G were disrupted by syringe passage. Intact parasites were pelleted at 1,400 g (1.4K P), and the supernatant, containing material from the vacuolar space and from disrupted parasites, was subjected to further differential centrifugation. Identical parasite equivalents from the 1.4 K P, 10 K P, 100 K P, and 100 K S samples were analyzed by SDS-PAGE immunoblot with antibodies to BAP. Only BAP is a soluble protein in the vacuolar space.