Table I.
Cell Cycle Progression in HeLa and PtK1 Cells Injected with Control Rabbit IgG or Anti-p55CD
| Injection/cell phase injected | n | No. of cells arrested at metaphase | Duration of metaphase in min (mean ± SD) | Duration of anaphase in min (mean ± SD) | Specific notes | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| HeLa | ||||||||||
| control rabbit IgG‡ | 10 | 0 | 15.2 ± 4.6 | 3.2 ± 0.9 | ||||||
| early/late prometaphase | ||||||||||
| anti-p55CDC‡ | 19 | 0 | 35.0 ± 30.7‖ | 5.7 ± 3.0 | ||||||
| early/late prometaphase | ||||||||||
| PtK1 | ||||||||||
| control rabbit IgG | 32 | 0 | 10.8 ± 4.7 | 9.3 ± 4.0 | ||||||
| late prophase/early metaphase | 3 arrested at early anaphase | |||||||||
| anti-p55CDC§ | 31 | 10 | 38.0 ± 20.7‖ | 23.3 ± 13.1‖ | 4 arrested at late anaphase | |||||
| late prophase/early metaphase | 5 without cytokinesis |
*
8.0 mg/ml in KCl-buffer.
‡
2.0 mg/ml in KCl-buffer.
§
1.0-2.5 mg/ml in KCl- or Tris-buffer.
‖
P < 0.01.