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. 1998 Jun 15;141(6):1301–1310. doi: 10.1083/jcb.141.6.1301

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Time course estrogen effect on the amount of the 104-kD protein present in the nuclear extract and coimmunoprecipitated with the estrogen receptor. SDS-PAGE followed by Western blot analysis with mAb 1032 to the major vault protein of nuclear extracts (a) or of proteins immunoprecipitated from nuclear extracts with the mAb 1603 to estrogen receptor (b) of cells incubated in the presence of 10 nM estradiol or 100 nM ICI182,780 for the indicated time. The arrows on the left side indicate the molecular weight (kD) of the band calculated from migration of standard proteins; the plots on the bottom are the densitometric analysis of the blots (•, estradiol; ▵, ICI182,780).