Figure 7.

Immunocytochemical analysis of cadherin-6 gene expression. Mouse bone marrow cells containing mononuclear hemopoietic cells and stromal cells were treated with 10 nM 1,25D₃ and 100 nM dexamethasone for 6 d. The cultures containing multinucleated osteoclast-like cells, mononuclear hemopoietic cells and stromal sells were then incubated with the polyclonal antibody to cadherin-6/2. Multinucleated osteoclast-like cells (arrows) and mononuclear cells (asterisks) with round (hemopoietic cells) or spindle (stromal cells) were positively stained by the cadherin-6/2 antibody (left) but not by the preimmune serum (right).