Figure 11.

(A) Sensitivity to endoglycosidase H and endoglycosidase F of DPP-IV and CEA from control and GalNAc-α-O-benzyl-treated HT29-RevMTX10⁻⁵ cells (day 21). The same quantity of cell homogenate (30 μg of proteins) treated as indicated was loaded in each lane. Western blot analysis of DPP-IV and CEA was performed with mAbs 4H3 and 517, respectively. The samples for each protein were run on the same gel in a different order. For clarity they have been rearranged in the figure. (B) Pulse-chase experiment with DPP-IV. Control and GalNAc-α-O-benzyl–treated cells (day 21) were labeled with [³⁵S]methionine for 15 min followed by a chase in complete medium containing unlabeled methionine. After the indicated periods of chase, DPP-IV was immunoprecipitated with mAb 4H3 and immunoprecipitates were subjected to SDS-PAGE.