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. 1998 Jun 15;141(6):1311–1322. doi: 10.1083/jcb.141.6.1311

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Effect of GalNAc-α-O-benzyl on the cellular distribution of polarized proteins in mucus-secreting HT-29 cells. Indirect immunofluorescence detection of polarized proteins in frozen cryostat sections of the cell layers from control (left column) and 2 mM GalNAc-α-O-benzyl– treated HT29-RevMTX10⁻⁶ cells (right column) analyzed after 21 d in culture. Sections of the cell layers were single labeled with antibodies against villin (a and b), ZO1 (c and d), glycoprotein GP120 (e and f), DPP-IV (g and h), MUC1 (i and j), and CEA (k and l). Bar, 40 μm.