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. 1998 Jun 15;141(6):1311–1322. doi: 10.1083/jcb.141.6.1311

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Reversibility of the effects of GalNAc-α-O-benzyl on cell morphology, distribution of DPP-IV, and mucus secretion. HT29-RevMTX10⁻⁶ cells were cultured until day 15 in the presence of 2 mM GalNAc-α-O-benzyl and then in drug-free medium. Cultures were analyzed on day 15 (a–c), and 5 d after removal of the drug: (d–f). Left column, thin sections of the cell layer; middle column, indirect immunofluorescence detection of DPP-IV with mAb 3/775/42; right column, indirect immunofluorescence detection of mucus with pAb L56/C in sections of the cell layer. Similar results for DPP-IV were observed as for MUC1 and CEA (data not shown). Bars: left column (16 μm); middle and right columns (80 μm).