Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Oct 5;143(1):35–47. doi: 10.1083/jcb.143.1.35

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

The structure of the PNC changes upon transcription inhibition by α-amanitin (an inhibitor of RNA polymerase II and/or III) in cells transiently expressing GFP– PTB (A). When double labeled with the Sm antibody (B), the extended PNC appears to form rosettes surrounding the round Sm clusters (C, inset). This change was not observed in cells that do not contain a PNC (D–F), suggesting that the change is specific to a reorganization of the PNC. When the distribution of GFP–PTB (G) and CUG-BP/hNab50 (H) was compared upon transcription inhibition by α-amanitin, a similar reorganization of the PNC in the nucleus was observed for both hnRNP proteins (G–I) in spite of the fact that the majority of the CUG-BP/hNab50 protein becomes predominantly localized in the cytoplasm (H). Bar, 10 μm.