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. 1998 Oct 5;143(1):35–47. doi: 10.1083/jcb.143.1.35

Figure 5.

Figure 5

Pre-mRNA processing factors are localized in the PNC. HeLa cells transiently expressing GFP–PTB (left column, arrows indicating the PNC) were immunolabeled with antibodies specifically recognizing several pre-mRNA processing factors as indicated (middle column, arrows showing the same nuclear regions containing these factors). The right column shows the colocalization of the PNC and the RNA processing factors. P80-coilin (bottom row), as a negative control, is not detected in the PNC. Bar, 10 μm.