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. 1998 Oct 5;143(1):207–215. doi: 10.1083/jcb.143.1.207

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Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity (A). MitoTracker Green FM shows exclusive punctate labeling (B). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria (yellow) as well as nonmitochondrial BAX immunoreactivity (red; C). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap (D).